HNMETG lysis buffer

Final concentrations:

• 50 mM HEPES/NaOH pH 7.5
• 150 mM NaCl
• 1.5 mM MgCl2
• 1 mM EGTA
• 1% Triton X-100
• 10% glycerol

For 200 ml:

• 10 ml 1 M HEPES/NaOH
• 6 ml 5 M NaCl
• 300 ul 1 M MgCl2
• 400 ul 0.5 M EGTA
• 2 ml Triton X-100
• 20 ml glycerol
• 161.3 ml H20 (to 200 ml)