HNMETG lysis buffer

Final concentrations:

  • 50 mM HEPES/NaOH pH 7.5
  • 150 mM NaCl
  • 1.5 mM MgCl2
  • 1 mM EGTA
  • 1% Triton X-100
  • 10% glycerol

For 200 ml:

  • 10 ml 1 M HEPES/NaOH
  • 6 ml 5 M NaCl
  • 300 ul 1 M MgCl2
  • 400 ul 0.5 M EGTA
  • 2 ml Triton X-100
  • 20 ml glycerol
  • 161.3 ml H20 (to 200 ml)

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