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Buffer Recipes
10x Running buffer for Tris-Glycine SDS-PAGE
4x SDS Protein Sample Buffer
Coomassie blue staining solution for polyacrylamide gels
HNMETG lysis buffer
mRIPA lysis buffer
Plasmid sequences
pCDNA3.1-human-ub-wt
Protocols
Directional PCR Subcloning
Purification of GST-fusion proteins from E. coli
Tutorials
Easy single-primer site-directed mutagenesis
Coomassie blue staining solution for polyacrylamide gels
Final Concentrations:
40% methanol
10% acetic acid
50% H20
0.1% (w/v) coomassie blue dye
For 500 ml:
200 ml methanol
50 ml acetic acid
250 ml H20
0.5 g coomassie blue
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Buffer Recipes
10x Running buffer for Tris-Glycine SDS-PAGE
4x SDS Protein Sample Buffer
Coomassie blue staining solution for polyacrylamide gels
HNMETG lysis buffer
mRIPA lysis buffer
Plasmid sequences
pCDNA3.1-human-ub-wt
Protocols
Directional PCR Subcloning
Purification of GST-fusion proteins from E. coli
Tutorials
Easy single-primer site-directed mutagenesis
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